Our Publications

Novel Methods examples:

  • Applications of simultaneous PCNA- & TUNEL-labeling to evaluate testicular toxicity suggests apoptosis is more sensitive to toxicity than proliferation. Biotechnic and Histochemistry, 85:195-204, 2010.
  • Application of triple-label immunohistochemistry to characterize inflammation in Alzheimer’s disease brains. Biotechnic & Histochemistry 76(2):97-106, 2001.
  • A simultaneous histochemical and immunohistochemical staining protocol to evaluate 4 differently stained cell types in restenosis. Biotechnic & Histochemistry 74(4):172- 180, 1999.

Assay Development examples:

  • Collagenase pre-digestion on paraffin sections enhances collagen immunohistochemical detection without distorting tissue morphology. Biotechnique & Histochemistry 79(2):55-64, 2004.
  • The use of formic acid to embellish amyloid plaque detection in Alzheimer’s disease tissues misguides key observations. Neuroscience Letters 342(2):114-118, 2003.
  • Epitope recovery immunohistochemical methods insure intracellular localization of Abeta42 within Alzheimer’s disease pyramidal neurons. Neuroscience Letters 333(3):163-166, 2002.

Semi-Quantitative Assay examples:

  • PCNA-indexing as a preclinical immunohistochemical biomarker for testicular toxicity. Biotechnic & Histochemistry, 83(5):211-220, 2008.
  • Lack of neurokinin-1 receptor expression affects tissue mast cell numbers but not their spatial relationship with nerves. Am J Physiology 288(2):491-500, 2005.
  • Activity Dependent Neurotrophic Factor-9 (ADNF-9) and NAP Promote Neurite Outgrowth in Rat Hippocampal and Cortical Cultures. J Molecular Neuroscience 25(3); 225-38, 2005.
  • Administration of a Potent Antagonist of Protease-Activated Receptor-1 (PAR-1) attenuates vascular restenosis following angioplasty in rats. J Pharm & Exp Therapeutics 298(1):34-42, 2001.
  • Increased expression of serine palmitoyltransferase (SPT) in balloon-injured rat carotid artery. Arteriosclerosis, Thrombosis, and Vascular Biology 86:1320-1326, 2001.

Target Distribution/Discovery examples:

  • Localization of Protease-activated receptors -1 and -2 in human mast cells: Indications for mast cell amplification cascade. Biotechnic & Histochemistry 75(2):85-90, 2000.
  • Expression of PDE11A in normal and malignant human tissues. J Histochem & Cytochem 53:895-903, 2005.
  • Elevated serine palmitoyltransferase expression in proliferating fibroblasts, transformed cell lines, and human tumors. J Histochem Cytochem 51(6):715-726, 2003.
  • Characterization of serine palmitoyltransferase in normal human tissues. J Histochem Cytochem 51(5):687-696, 2003.
  • Molecular cloning, gene structure and tissue distribution of putative human voltage gated calcium channel alpha2beta4 subunit. Mol Pharmacology 62(3):485-496, 2002.

Validation examples:

  • TRPM8 deletion increases cold-tolerance, precludes neuropathy-induced cold allodynia and prevents icilin-induced jumping in mice. Neuron, 54(3):379-386, 2007.
  • Application of immunohistochemistry and quantitative image analysis to demonstrate in vivo inhibitory activities of PDGF receptor kinase compounds in experimental models of tumor angiogenesis. Molecular Cancer Therapeutics 4(8):1198-1204, 2005.
  • Acute colitis induction by oil of mustard results in later development of IBS-like accelerated upper GI transit in mice. Am J Physiology Gastrointest Liver Physiol 288(6):G1266-73, 2005.
  • Vanilloid receptor 1 antagonists attenuate disease severity in dextran sulfate sodium induced colitis in mice.  Neurogastroenterology and Motility 16(6), 811-8, 2004.